An ultracentrifugal study of the dissociation of human carboxyhemoglobin

• Main Author: Hutchinson, William Day
• Format: Others
• Published: 1957
• Online Access: https://thesis.library.caltech.edu/5742/1/Hutchinson_wd_1957.pdf; Hutchinson, William Day (1957) An ultracentrifugal study of the dissociation of human carboxyhemoglobin. Master's thesis, California Institute of Technology. doi:10.7907/W8JC-7T49. https://resolver.caltech.edu/CaltechTHESIS:04262010-094002832 <https://resolver.caltech.edu/CaltechTHESIS:04262010-094002832>

The dissociation of human carboxyhemoglobin has been investigated by means of sedimentation velocity measurements. Sedimentation studies on this protein have been extended to concentrations as low as 0.002% by the use of absorption optics. Hemoglobin was observed to dissociate increasingly as the pH was changed from 7 to 5. Apparent dissociation constants at each pH have been calculated. Values of the apparent specific volume of human carboxyhemoglobin have been found.