Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology
<p>This work details the development of bioanalytical tools for use in rapid diagnostics (Chapters 2-4) and in the study of physiology (Chapters 5-6). This research harness the power of real-time, singlemolecule microfluidics to study loop-mediated isothermal amplification in urinary tract inf...
| id |
ndltd-CALTECH-oai-thesis.library.caltech.edu-11559 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-CALTECH-oai-thesis.library.caltech.edu-115592020-01-31T03:02:30Z Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology Rolando, Justin Charles <p>This work details the development of bioanalytical tools for use in rapid diagnostics (Chapters 2-4) and in the study of physiology (Chapters 5-6). This research harness the power of real-time, singlemolecule microfluidics to study loop-mediated isothermal amplification in urinary tract infections (Chapter 2), chlamydia (Chapter 3), and gonorrhea (Chapter 4). In Chapter 5, non-reactive beads are designed and optimized to study the impact of polymers on murine gastrointestinal mucosa. Chapter 6 details the implementation of a mass spectrometry method to quantify bile acids and investigate their interaction with the microbiota in the murine gastro-intestinal tract.</p> 2019 Thesis NonPeerReviewed application/pdf https://thesis.library.caltech.edu/11559/13/Rolando_Final_v2.pdf https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023 Rolando, Justin Charles (2019) Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/99NT-WP75. https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023 <https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023> https://thesis.library.caltech.edu/11559/ |
| collection |
NDLTD |
| format |
Others
|
| sources |
NDLTD |
| description |
<p>This work details the development of bioanalytical tools for use in rapid diagnostics (Chapters 2-4) and in the study of physiology (Chapters 5-6). This research harness the power of real-time, singlemolecule microfluidics to study loop-mediated isothermal amplification in urinary tract infections (Chapter 2), chlamydia (Chapter 3), and gonorrhea (Chapter 4). In Chapter 5, non-reactive beads are designed and optimized to study the impact of polymers on murine gastrointestinal mucosa. Chapter 6 details the implementation of a mass spectrometry method to quantify bile acids and investigate their interaction with the microbiota in the murine gastro-intestinal tract.</p> |
| author |
Rolando, Justin Charles |
| spellingShingle |
Rolando, Justin Charles Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology |
| author_facet |
Rolando, Justin Charles |
| author_sort |
Rolando, Justin Charles |
| title |
Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology |
| title_short |
Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology |
| title_full |
Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology |
| title_fullStr |
Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology |
| title_full_unstemmed |
Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology |
| title_sort |
bioanalytical tools to develop rapid diagnostics and study physiology |
| publishDate |
2019 |
| url |
https://thesis.library.caltech.edu/11559/13/Rolando_Final_v2.pdf Rolando, Justin Charles (2019) Bioanalytical Tools to Develop Rapid Diagnostics and Study Physiology. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/99NT-WP75. https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023 <https://resolver.caltech.edu/CaltechTHESIS:05292019-112017023> |
| work_keys_str_mv |
AT rolandojustincharles bioanalyticaltoolstodeveloprapiddiagnosticsandstudyphysiology |
| _version_ |
1719310582439477248 |