Experiments on the Nutrition of Isolated Roots
<p>1. The history of plant tissue culture is outlined.</p> <p>2. The technique of root cultures is reinvestigated from several aspects and some changes are incorporated for the present work.</p> <p>3. The section of vitamin B<sub>1</sub> as a root grow...
| Summary: | <p>1. The history of plant tissue culture is outlined.</p>
<p>2. The technique of root cultures is reinvestigated from several aspects and some changes are incorporated for the present work.</p>
<p>3. The section of vitamin B<sub>1</sub> as a root growth hormone is examined, and its presence is found to be closely correlated with the growth rate, rate of cell divisions, and extent of meristem in isolated roots.</p>
<p>4. A second growth factor of pea roots present in yeast extract is found not to be among the micorelements of plant nutrition nor among the amino acids.</p>
<p>5. Nicotinic acid is found to be the second growth factor of pea roots. With vitamin B<sub>1</sub> it is capable of supporting a higher rate of growth for pea roots than can yeast extract.</p>
<p>6. Evidence is presented to show that inositol, phosphate, and optimum hydrogen ion concentration may have promotice effects on the growth of pea roots.</p>
<p>7. A number of experiments are described which indicate that it may be impossible to establish a clone of pea roots.</p>
<p>8. Preliminary experiments on the culture of excised anthers are described, and the high activity of embryo extracts on anther growth is noted.</p> |
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