DNA sequence analysis of the rDNA ITS 1/2 region in the evolutionary delineation of the Pilobolaceae

• Main Author: Gilmore, Luther Martin
• Other Authors: Ruch, Donald Gene, 1948-
• Format: Others
• Published: 2011
• Subjects: Pilobolus > Classification.; Pilobolus > Evolution.; Ribosomes.
• Online Access: http://cardinalscholar.bsu.edu/handle/handle/188147; http://liblink.bsu.edu/uhtbin/catkey/1348863

This project represents an initial examination of the evolutionary history of the genus Pilobolus, and provides a starting point for developing a method to accurately classify members of this genus using molecular genetics. The data analysis presented in this paper suggests a potential evolutionary path in which P. umbonatus and P. sphaerosporus diverged (along a common path) from an original generic ancestor early, but independently, from the evolutionary course taken by P. kleinii. Both Maximum Likelihood and parsimony analyses concur in the branch patterns. Additionally, formation of species-specific clades between samples of P. sphaerosporus, P. kleinii and P. umbonatus suggests that there may be markers present in the ITS sequence that can be used for the development of a molecular test for the classification of species within the genus Pilobolus.Ball State UniversityMuncie, IN 47306 === Department of Biology