Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.

Gram-negative infection has been linked to hospital water although few studies have examined whether water systems are reservoirs of nosocomial pathogens. This study investigated longitudinal recovery of the opportunistic pathogens Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Acinetobact...

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Main Author: Wright, Claire Louise
Other Authors: Snelling, Anna M.
Language:en
Published: University of Bradford 2013
Subjects:
Online Access:http://hdl.handle.net/10454/5692
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spelling ndltd-BRADFORD-oai-bradscholars.brad.ac.uk-10454-56922019-08-31T03:03:02Z Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention. Wright, Claire Louise Snelling, Anna M. Water-associated-pathogens Haematology Opportunistic Gram-negative Hospital acquired infections Immunocompromised Stenotrophomonas maltophilia Pseudomonas aeruginosa Water microbiology Filtration Nosocomial pathogens Point-Of-Use Filtration (POU-F) Gram-negative infection has been linked to hospital water although few studies have examined whether water systems are reservoirs of nosocomial pathogens. This study investigated longitudinal recovery of the opportunistic pathogens Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Acinetobacter baumannii from water outlets of a haematology unit and evaluated Point-Of-Use Filtration (POU-F) as a control measure. In a two-year double cross-over trial, water samples and swabs were taken weekly from 39 showers/taps on the unit. Four study phases alternated between non-filtered (Phases 1 & 3), and filtered outlets (Phases 2 & 4) using Pall AquasafeTM 14-day filters. In Phases 1 & 3; 99% of 1396 samples yielded bacterial growth, with colonies generally too numerous to count. Target species were isolated from 22% of water samples (P. aeruginosa 14%; S. maltophilia 10%) and 10% of swabs. P. aeruginosa was particularly associated with handwash stations and S. maltophilia with showers. A. baumannii was not isolated. With POU-F; 22% of 1242 samples yielded bacterial growth (mean CFU/100ml ,4.6). S. maltophilia was isolated only once from water but never from outlet swabs. PCR typing identified clusters of isolates colonizing different outlets over time but no clear association between water and patient isolates was identified. The incidence of Gram negative infections remained low throughout the study. Without POU-F, water from taps/showers represented a source of bacteria including the target species. POU-F substantially reduced the frequency and number of target species from every outlet, and merits further investigation as an intervention to protect immunocompromised patients from opportunistic pathogens. School of Life Sciences, University of Bradford and Pall Medical (Pall Europe Ltd) 2013-11-21T15:44:47Z 2013-11-21T15:44:47Z 2013-11-21 2012 Thesis doctoral PhD http://hdl.handle.net/10454/5692 en <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><img alt="Creative Commons License" style="border-width:0" src="http://i.creativecommons.org/l/by-nc-nd/3.0/88x31.png" /></a><br />The University of Bradford theses are licenced under a <a rel="license" href="http://creativecommons.org/licenses/by-nc-nd/3.0/">Creative Commons Licence</a>. University of Bradford School of Medical Sciences
collection NDLTD
language en
sources NDLTD
topic Water-associated-pathogens
Haematology
Opportunistic
Gram-negative
Hospital acquired infections
Immunocompromised
Stenotrophomonas maltophilia
Pseudomonas aeruginosa
Water microbiology
Filtration
Nosocomial pathogens
Point-Of-Use Filtration (POU-F)
spellingShingle Water-associated-pathogens
Haematology
Opportunistic
Gram-negative
Hospital acquired infections
Immunocompromised
Stenotrophomonas maltophilia
Pseudomonas aeruginosa
Water microbiology
Filtration
Nosocomial pathogens
Point-Of-Use Filtration (POU-F)
Wright, Claire Louise
Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
description Gram-negative infection has been linked to hospital water although few studies have examined whether water systems are reservoirs of nosocomial pathogens. This study investigated longitudinal recovery of the opportunistic pathogens Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Acinetobacter baumannii from water outlets of a haematology unit and evaluated Point-Of-Use Filtration (POU-F) as a control measure. In a two-year double cross-over trial, water samples and swabs were taken weekly from 39 showers/taps on the unit. Four study phases alternated between non-filtered (Phases 1 & 3), and filtered outlets (Phases 2 & 4) using Pall AquasafeTM 14-day filters. In Phases 1 & 3; 99% of 1396 samples yielded bacterial growth, with colonies generally too numerous to count. Target species were isolated from 22% of water samples (P. aeruginosa 14%; S. maltophilia 10%) and 10% of swabs. P. aeruginosa was particularly associated with handwash stations and S. maltophilia with showers. A. baumannii was not isolated. With POU-F; 22% of 1242 samples yielded bacterial growth (mean CFU/100ml ,4.6). S. maltophilia was isolated only once from water but never from outlet swabs. PCR typing identified clusters of isolates colonizing different outlets over time but no clear association between water and patient isolates was identified. The incidence of Gram negative infections remained low throughout the study. Without POU-F, water from taps/showers represented a source of bacteria including the target species. POU-F substantially reduced the frequency and number of target species from every outlet, and merits further investigation as an intervention to protect immunocompromised patients from opportunistic pathogens. === School of Life Sciences, University of Bradford and Pall Medical (Pall Europe Ltd)
author2 Snelling, Anna M.
author_facet Snelling, Anna M.
Wright, Claire Louise
author Wright, Claire Louise
author_sort Wright, Claire Louise
title Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
title_short Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
title_full Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
title_fullStr Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
title_full_unstemmed Investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
title_sort investigation of the prevalence of opportunistic gram negative pathogens in the water supply of a haematology unit, and the application of point-of-use filtration as an intervention.
publisher University of Bradford
publishDate 2013
url http://hdl.handle.net/10454/5692
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