Lipidomics of polyunsaturated fatty acid-derived oxygenated metabolites

• Main Authors: Nicolaou, Anna, Massey, Karen A.
• Language: en
• Published: 2011
• Subjects: Lipidomics; Fatty Acids; Mass Spectrometry; Polyunsaturated Fatty Acids; PUFA; Cytochrome P450; Eicosanoid; LOX; COX; Cyclo-Oxygenase; Lipoxygenase
• Online Access: http://hdl.handle.net/10454/5230

No === Nutritionally important PUFAs (polyunsaturated fatty acids) mediate some of their bioactivities through formation of oxygenated metabolites. These bioactive lipids are formed by COX (cyclo-oxygenase), LOX (lipoxygenase) and cytochrome-P450-catalysed reactions, as well as non-enzymatic lipid peroxidation. These reactions produce numerous species, some of which can be formed through more than one pathway. MS-based lipidomics offers the selectivity and sensitivity required for qualitative and quantitative analysis of multiple lipid species, in a variety of biological systems, and can facilitate the study of these mediators.