The dietary flavonol quercetin ameliorates angiotensin II-induced redox signaling imbalance in a human unbilical vein endothelial cell model of endothelial dysfunction via ablation of p47phox expression

• Main Authors: Jones, Huw S., Gordon, A., Magwensi, S.G., Naseem, K., Atkin, S.L., Courts, F.L.
• Language: en
• Published: Wiley 2020
• Subjects: Endothelial cells; NADPH oxidase; Nitric oxide; Quercetin; Superoxide
• Online Access: http://hdl.handle.net/10454/17790

Yes === Quercetin is reported to reduce blood pressure in hypertensive but not normotensive humans, but the role of endothelial redox signaling in this phenomenon has not been assessed. This study investigated the effects of physiologically obtainable quercetin concentrations in a human primary cell model of endothelial dysfunction in order to elucidate the mechanism of action of its antihypertensive effects. Angiotensin II (100 nM, 8 h) induced dysfunction, characterized by suppressed nitric oxide availability (85 ± 4% p<0.05) and increased superoxide production (136 ± 5 %, p<0.001). These effects were ablated by an NADPH oxidase inhibitor. Quercetin (3 μM, 8 h) prevented angiotensin II induced changes in nitric oxide and superoxide levels, but no effect upon nitric oxide or superoxide in control cells. The NADPH oxidase subunit p47(phox) was increased at the mRNA and protein levels in angiotensin II-treated cells (130 ± 14% of control, p<0.05), which was ablated by quercetin co-treatment. Protein kinase C activity was increased after angiotensin II treatment (136 ± 51%), however this was unaffected by quercetin co-treatment. Physiologically obtainable quercetin concentrations are capable of ameliorating angiotensin II-induced endothelial nitric oxide and superoxide imbalance via protein kinase C-independent restoration of p47(phox) gene and protein expression. === Innovate UK and Boots Pharmaceuticals