Streamlined Extract Preparation for E. coli-Based Cell-Free Protein Synthesis and Rapid Site-Specific Incorporation of Unnatural Amino Acids in Proteins

Streamlined Extract Preparation for E. coli-Based Cell-Free Protein Synthesis and Rapid Site-Specific Incorporation of Unnatural Amino Acids in Proteins

This thesis reports the viability of E. coli cell extracts prepared using equipment that is both common to biotechnology laboratories and able to process small volume samples and expression of proteins containing unnatural amino acids (UAAs) at higher level using PCR amplified linear DNA templates (...

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Bibliographic Details
Main Author: Shrestha, Prashanta
Format: Others
Published: BYU ScholarsArchive 2012
Subjects:
Prashanta Shrestha
cell-free protein synthesis
in vitro protein synthesis
unnatural amino acids
para-proparglyoxyphenylalanine (pPa)
cell lysis
cell extract
bead milling
sonication
RecBCD
exonucleases
small molecules
linear expression templates
linear DNA
PCR
transcription
translation
Chemical Engineering
Online Access:https://scholarsarchive.byu.edu/etd/3917
https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=4916&context=etd

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