Structure Elucidation of Bioactive Compounds Isolated from Endophytes of Alstonia scholaris and Acmena graveolens

• Main Author: Hundley, Nicholas James
• Format: Others
• Published: BYU ScholarsArchive 2005
• Subjects: endophtye; endophytes; endophytic (endophyt*); fungi; fungal; symbiosis; rDNA; ribosomal DNA; fungal taxonomy; ITS; cytochalasins; cytochalasans; xylobovide; fusaric acid; dehydrofusaric acid; xylaria; xylariaceae; rosellinia necatrix; taxol; HPLC; NMR; GC; gas chromatography; MS; mass spectrometry (mass spec*); IR; infrared spectroscopy; PCR; natural products; ethnobotany; ethnomedicine; COSY; HMQC; HMBC; Biochemistry; Chemistry
• Online Access: https://scholarsarchive.byu.edu/etd/661; https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=1660&context=etd

Alstonia scholaris is an evergreen tree native to Southeast Asia and Australia. It is commonly used as a medicinal plant throughout these regions. In the present study, an endophyte of the genus Xylaria was isolated from a stem of Alstonia scholaris, its mycelia and exudate extracted, and the extract assayed for growth inhibition of HeLa cancer cells in vitro. Several known compounds were isolated and identified based on NMR, infrared, and mass spectral data. The compounds identified are 19,20-epoxycytochalasin C; 19,20epoxycytochalasin D; and xylobovide. Two other compounds, fusaric acid and dehydrofusaric acid, were discovered in an endophyte of the Hypocreales family inhabiting the plant Acmena Graveolens.