Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers

• Main Authors: Gaylord, Shonda T. (Author), Ngan, Kevin C. (Author), Dumont Milutinovic, Milena (Author), Kwong, Gabriel A. (Contributor), Bhatia, Sangeeta N. (Contributor), Walt, David R. (Author), Warren, Andrew David (Contributor)
• Other Authors: Harvard University- (Contributor)
• Format: Article
• Language: English
• Published: American Chemical Society (ACS), 2015-11-10T15:08:02Z.
• Subjects: Article
• Online Access: Get fulltext

The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for "microdosing."
National Science Foundation (U.S.). Graduate Research Fellowship
National Institutes of Health (U.S.) (Ruth L. Kirschstein National Research Service Award F32CA159496-02)
Burroughs Wellcome Fund (Career Award at the Scientific Interface)
National Cancer Institute (U.S.) (Koch Institute Support (Core) Grant P30-CA14051)
David H. Koch Institute for Integrative Cancer Research at MIT (Frontier Research Program)