CRISPR transcriptional repression devices and layered circuits in mammalian cells

CRISPR transcriptional repression devices and layered circuits in mammalian cells

A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of...

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Bibliographic Details
Main Authors: Kiani, Samira (Contributor), Beal, Jacob (Author), Huh, Jin (Contributor), Xie, Zhen (Author), Li, Yinqing (Author), Weiss, Ron (Contributor), Ebrahimkhani, Mohammad Reza (Contributor), Hall, Richard N. (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Synthetic Biology Center (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2015-10-30T17:33:13Z.
Subjects:
Article
Online Access:Get fulltext
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042 |a dc 
100 1 0 |a Kiani, Samira  |e author 
100 1 0 |a Massachusetts Institute of Technology. Department of Biological Engineering  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Synthetic Biology Center  |e contributor 
100 1 0 |a Kiani, Samira  |e contributor 
100 1 0 |a Ebrahimkhani, Mohammad Reza  |e contributor 
100 1 0 |a Huh, Jin  |e contributor 
100 1 0 |a Hall, Richard N.  |e contributor 
100 1 0 |a Weiss, Ron  |e contributor 
700 1 0 |a Beal, Jacob  |e author 
700 1 0 |a Huh, Jin  |e author 
700 1 0 |a Xie, Zhen  |e author 
700 1 0 |a Li, Yinqing  |e author 
700 1 0 |a Weiss, Ron  |e author 
700 1 0 |a Ebrahimkhani, Mohammad Reza  |e author 
700 1 0 |a Hall, Richard N.  |e author 
245 0 0 |a CRISPR transcriptional repression devices and layered circuits in mammalian cells 
260 |b Nature Publishing Group,   |c 2015-10-30T17:33:13Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/99528 
520 |a A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes. 
520 |a National Institutes of Health (U.S.) (Grant 5R01CA155320-04) 
520 |a National Institutes of Health (U.S.) (Grant P50 GM098792) 
520 |a Korea (South). Ministry of Science, Information and Communication Technolgy. Intelligent Synthetic Biology Center of Global Frontier Project (2013M3A6A8073557) 
546 |a en_US 
655 7 |a Article 
773 |t Nature Methods 

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