CRISPR transcriptional repression devices and layered circuits in mammalian cells

• Main Authors: Kiani, Samira (Contributor), Beal, Jacob (Author), Huh, Jin (Contributor), Xie, Zhen (Author), Li, Yinqing (Author), Weiss, Ron (Contributor), Ebrahimkhani, Mohammad Reza (Contributor), Hall, Richard N. (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Synthetic Biology Center (Contributor)
• Format: Article
• Language: English
• Published: Nature Publishing Group, 2015-10-30T17:33:13Z.
• Subjects: Article
• Online Access: Get fulltext

A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
National Institutes of Health (U.S.) (Grant 5R01CA155320-04)
National Institutes of Health (U.S.) (Grant P50 GM098792)
Korea (South). Ministry of Science, Information and Communication Technolgy. Intelligent Synthetic Biology Center of Global Frontier Project (2013M3A6A8073557)