Efficient CRISPR/Cas9-mediated genome editing in P. falciparum

Efficient CRISPR/Cas9-mediated genome editing in P. falciparum

Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmo...

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Bibliographic Details
Main Authors: Zhang, Feng (Contributor), Wagner, Jeffrey C. (Contributor), Platt, Randall Jeffrey (Contributor), Goldfless, Stephen J. (Contributor), Niles, Jacquin (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2015-10-29T15:05:06Z.
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