Clustered Dynamics of Inhibitory Synapses and Dendritic Spines in the Adult Neocortex

• Main Authors: Chen, Jerry L. (Contributor), Villa, Katherine Leigh (Contributor), Cha, Jae Won (Contributor), So, Peter T. C. (Contributor), Kubota, Yoshiyuki (Author), Nedivi, Elly (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Massachusetts Institute of Technology. Department of Mechanical Engineering (Contributor), Picower Institute for Learning and Memory (Contributor)
• Format: Article
• Language: English
• Published: Elsevier B.V., 2014-11-24T17:01:53Z.
• Subjects: Article
• Online Access: Get fulltext

A key feature of the mammalian brain is its capacity to adapt in response to experience, in part by remodeling of synaptic connections between neurons. Excitatory synapse rearrangements have been monitored in vivo by observation of dendritic spine dynamics, but lack of a vital marker for inhibitory synapses has precluded their observation. Here, we simultaneously monitor in vivo inhibitory synapse and dendritic spine dynamics across the entire dendritic arbor of pyramidal neurons in the adult mammalian cortex using large-volume, high-resolution dual-color two-photon microscopy. We find that inhibitory synapses on dendritic shafts and spines differ in their distribution across the arbor and in their remodeling kinetics during normal and altered sensory experience. Further, we find inhibitory synapse and dendritic spine remodeling to be spatially clustered and that clustering is influenced by sensory input. Our findings provide in vivo evidence for local coordination of inhibitory and excitatory synaptic rearrangements.
National Eye Institute (RO1 EY017656)
National Eye Institute (RO1 EY011894)
Singapore-MIT Alliance (Singapore-MIT Alliance-2)
Singapore-MIT Alliance for Research and Technology
Japan. Ministry of Education, Culture, Sports, Science and Technology (Grant-in-aid for Scientific Research on Innovative Areas: Neural creativity for communication (No.4103) (22120518))