Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells

• Main Authors: Shalek, Alex K. (Author), Satija, Rahul (Author), Adiconis, Xian (Author), Gertner, Rona S. (Author), Gaublomme, Jellert T. (Author), Raychowdhury, Raktima (Author), Schwartz, Schraga (Author), Yosef, Nir (Author), Malboeuf, Christine (Author), Trombetta, John J. (Author), Gennert, David (Author), Gnirke, Andreas (Author), Goren, Alon (Author), Hacohen, Nir (Author), Levin, Joshua Z. (Author), Park, Hongkun (Author), Regev, Aviv (Contributor), Lu, Diana, Ph. D. Massachusetts Institute of Technology (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor)
• Format: Article
• Language: English
• Published: Nature Publishing Group, 2014-03-14T17:23:08Z.
• Subjects: Article
• Online Access: Get fulltext

Recent molecular studies have shown that, even when derived from a seemingly homogenous population, individual cells can exhibit substantial differences in gene expression, protein levels and phenotypic output1, 2, 3, 4, 5, with important functional consequences4, 5. Existing studies of cellular heterogeneity, however, have typically measured only a few pre-selected RNAs1, 2 or proteins5, 6 simultaneously, because genomic profiling methods3 could not be applied to single cells until very recently7, 8, 9, 10. Here we use single-cell RNA sequencing to investigate heterogeneity in the response of mouse bone-marrow-derived dendritic cells (BMDCs) to lipopolysaccharide. We find extensive, and previously unobserved, bimodal variation in messenger RNA abundance and splicing patterns, which we validate by RNA-fluorescence in situ hybridization for select transcripts. In particular, hundreds of key immune genes are bimodally expressed across cells, surprisingly even for genes that are very highly expressed at the population average. Moreover, splicing patterns demonstrate previously unobserved levels of heterogeneity between cells. Some of the observed bimodality can be attributed to closely related, yet distinct, known maturity states of BMDCs; other portions reflect differences in the usage of key regulatory circuits. For example, we identify a module of 137 highly variable, yet co-regulated, antiviral response genes. Using cells from knockout mice, we show that variability in this module may be propagated through an interferon feedback circuit, involving the transcriptional regulators Stat2 and Irf7. Our study demonstrates the power and promise of single-cell genomics in uncovering functional diversity between cells and in deciphering cell states and circuits.
National Institutes of Health (U.S.) (NIH Postdoctoral Fellowship (1F32HD075541-01))
Charles H. Hood Foundation (Postdoctoral Fellowship)
National Institutes of Health (U.S.) (NIH grant U54 AI057159)
National Institutes of Health (U.S.) (NIH New Innovator Award (DP2 OD002230))
National Institutes of Health (U.S.) (NIH CEGS Award (1P50HG006193-01))
National Institutes of Health (U.S.) (NIH Pioneer Awards (5DP1OD003893-03))
National Institutes of Health (U.S.) (NIH Pioneer Awards (DP1OD003958-01))
Broad Institute of MIT and Harvard
Broad Institute of MIT and Harvard (Klarman Cell Observatory)