FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions

FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions

The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the...

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Bibliographic Details
Main Authors: Imperiali, Barbara (Contributor), Socher, Elke Ricarda (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: Wiley Blackwell, 2014-01-27T18:03:39Z.
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Summary:The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals.
National Institutes of Health (U.S.) (Grant R01 EB010246)

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