Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs

Weak seed-pairing stability and high target-site abundance decrease the proficiency of lsy-6 and other microRNAs

Most metazoan microRNAs (miRNAs) target many genes for repression, but the nematode lsy-6 miRNA is much less proficient. Here we show that the low proficiency of lsy-6 can be recapitulated in HeLa cells and that miR-23, a mammalian miRNA, also has low proficiency in these cells. Reporter results and...

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Bibliographic Details
Main Authors: Garcia, David M. (Contributor), Baek, Daehyun (Contributor), Shin, Chanseok (Contributor), Bell, George W. (Contributor), Grimson, Andrew (Contributor), Bartel, David (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2013-12-19T17:04:25Z.
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