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01896 am a22002773u 4500 |
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79074 |
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|a dc
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|a Mu, C. Jenny
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|a Harvard University-
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|a Massachusetts Institute of Technology. Department of Chemical Engineering
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|a Langer, Robert
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|a Mu, C. Jenny
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|a LaVan, David A.
|e author
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|a Langer, Robert
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|a Zetter, Bruce R.
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|a Self-Assembled Gold Nanoparticle Molecular Probes for Detecting Proteolytic Activity
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|b American Chemical Society,
|c 2013-06-06T19:42:57Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/79074
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|a Target-activatable fluorogenic probes based on gold nanoparticles (AuNPs) functionalized with self-assembled heterogeneous monolayers of dye-labeled peptides and poly(ethylene glycol) have been developed to visualize proteolytic activity in vivo. A one-step synthesis strategy that allows simple generation of surface-defined AuNP probe libraries is presented as a means of tailoring and evaluating probe characteristics for maximal fluorescence enhancement after protease activation. Optimal AuNP probes targeted to trypsin and urokinase-type plasminogen activator required the incorporation of a dark quencher to achieve 5- to 8-fold signal amplification. These probes exhibited extended circulation time in vivo and high image contrast in a mouse tumor model.
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|a National Institutes of Health (U.S.) (NIH grant CA119402)
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|a National Institutes of Health (U.S.) (grant DE013023)
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|a National Institutes of Health (U.S.) (grant DE016516)
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|a National Science Foundation (U.S.). Materials Research Science and Engineering Centers (Program) (award number DMR-08-19762)
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|a en_US
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|a Article
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|t ACS Nano
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