Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

• Main Authors: Luo, Yuan (Contributor), Zervantonakis, Ioannis (Contributor), Oh, Se Baek (Contributor), Kamm, Roger Dale (Contributor), Barbastathis, George (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Massachusetts Institute of Technology. Department of Mechanical Engineering (Contributor)
• Format: Article
• Language: English
• Published: SPIE, 2013-05-01T17:56:01Z.
• Subjects: Article
• Online Access: Get fulltext

A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
National Institutes of Health (U.S.) (NIH-RO1CA134424)
Singapore-MIT Alliance for Research and Technology Center (SMART 015824-039)