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|a Charoenlap, Nisanart
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Division of Comparative Medicine
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|a Fox, James G.
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|a Fox, James G.
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|a Shen, Zeli
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|a McBee, Megan E.
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|a Muthupalani, Sureshkumar
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|a Wogan, Gerald N.
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|a Schauer, David B.
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|a Shen, Zeli
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|a McBee, Megan E.
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|a Muthupalani, Sureshkumar
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|a Wogan, Gerald N.
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|a Fox, James G.
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|a Schauer, David B.
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|a lkyl Hydroperoxide Reductase Is Required for Helicobacter cinaedi Intestinal Colonization and Survival under Oxidative Stress in BALB/c and BALB/c Interleukin-10-/- Mice
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|b American Society for Microbiology,
|c 2013-04-03T20:21:02Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/78273
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|a Helicobacter cinaedi, a common human intestinal bacterium, has been implicated in various enteric and systemic diseases in normal and immunocompromised patients. Protection against oxidative stress is a crucial component of bacterium-host interactions. Alkyl hydroperoxide reductase C (AhpC) is an enzyme responsible for detoxification of peroxides and is important in protection from peroxide-induced stress. H. cinaedi possesses a single ahpC, which was investigated with respect to its role in bacterial survival during oxidative stress. The H. cinaedi ahpC mutant had diminished resistance to organic hydroperoxide toxicity but increased hydrogen peroxide resistance compared with the wild-type (WT) strain. The mutant also exhibited an oxygen-sensitive phenotype and was more susceptible to killing by macrophages than the WT strain. In vivo experiments in BALB/c and BALB/c interleukin-10 (IL-10)−/− mice revealed that the cecal colonizing ability of the ahpC mutant was significantly reduced. The mutant also had diminished ability to induce bacterium-specific immune responses in vivo, as shown by immunoglobulin (IgG2a and IgG1) serum levels. Collectively, these data suggest that H. cinaedi ahpC not only contributes to protecting the organism against oxidative stress but also alters its pathogenic properties in vivo.
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|a National Institutes of Health (U.S.) (grant R01CA067529)
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|a National Institutes of Health (U.S.) (grant R01DK052413)
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|a National Institutes of Health (U.S.) (grant R01RR032307)
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|a National Institutes of Health (U.S.) (grant P01CA26731)
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|a National Institutes of Health (U.S.) (grant P30ES002109)
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|a en_US
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|a Article
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|t Infection and Immunity
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