Quantitative cerebral blood flow with optical coherence tomography

Quantitative cerebral blood flow with optical coherence tomography

Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies of cerebral pathophysiology. Currently no accepted method exists for in vivo longitudinal monitoring of CBF with high resolution in rats and mice. Using three-dimensional Doppler Optical Coherence Tomography and...

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Bibliographic Details
Main Authors: Srinivasan, Vivek J. (Author), Sakadžic, Sava (Author), Gorczynska, Iwona (Contributor), Ruvinskaya, Svetlana (Author), Wu, Weicheng (Author), Fujimoto, James G. (Contributor), Boas, David A. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Massachusetts Institute of Technology. Research Laboratory of Electronics (Contributor)
Format: Article
Language:English
Published: Optical Society of America, 2012-09-07T15:29:33Z.
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Summary:Absolute measurements of cerebral blood flow (CBF) are an important endpoint in studies of cerebral pathophysiology. Currently no accepted method exists for in vivo longitudinal monitoring of CBF with high resolution in rats and mice. Using three-dimensional Doppler Optical Coherence Tomography and cranial window preparations, we present methods and algorithms for regional CBF measurements in the rat cortex. Towards this end, we develop and validate a quantitative statistical model to describe the effect of static tissue on velocity sensitivity. This model is used to design scanning protocols and algorithms for sensitive 3D flow measurements and angiography of the cortex. We also introduce a method of absolute flow calculation that does not require explicit knowledge of vessel angles. We show that OCT estimates of absolute CBF values in rats agree with prior measures by autoradiography, suggesting that Doppler OCT can perform absolute flow measurements in animal models.
National Institutes of Health (U.S.) (Grant number R01-NS057476)
National Institutes of Health (U.S.) (Grant number P01NS055104)
National Institutes of Health (U.S.) (Grant number P50NS010828)
ational Institutes of Health (U.S.) (Grant number K99NS067050)
National Institutes of Health (U.S.) (Grant number R01-CA075289-13)
United States. Air Force Office of Scientific Research (FA9550-07-1-0014)
United States. Dept. of Defense. Medical Free Electron Laser Program (FA9550-07-1-0101)

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