Opportunities in metabolic engineering to facilitate scalable alkaloid production

• Main Authors: Leonard, Effendi (Contributor), Runguphan, Weerawat (Contributor), O'Connor, Sarah Ellen (Contributor), Prather, Kristala L. Jones (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
• Format: Article
• Language: English
• Published: Nature Publishing Group, 2012-01-30T16:41:27Z.
• Subjects: Article
• Online Access: Get fulltext

Numerous drugs and drug precursors in the current pharmacopoeia originate from plant sources. The limited yield of some bioactive compounds in plant tissues, however, presents a significant challenge for large-scale drug development. Metabolic engineering has facilitated the development of plant cell and tissue systems as alternative production platforms that can be scaled up in a controlled environment. Nevertheless, effective metabolic engineering approaches and the predictability of genetic transformations are often obscured due to the myriad cellular complexities. Progress in systems biology has aided the understanding of genome-wide interconnectivities in plant-based systems. In parallel, the bottom-up assembly of plant biosynthetic pathways in microorganisms demonstrated the possibilities of a new means of production. In this Perspective, we discuss the opportunities and challenges of implementing metabolic engineering in various platforms for the synthesis of natural and unnatural plant alkaloids.
National Science Foundation (U.S.) (grant no. 0540879)
Synthetic Biology Engineering Research Center
Massachusetts Institute of Technology. Energy Initiative (Grant 6917278)
National Institutes of Health (U.S.) (GM074820)
National Science Foundation (U.S.) (MCB-0719120)