Zebrafish promoter microarrays identify actively transcribed embryonic genes

• Main Authors: Wardle, Fiona C (Author), Odom, Duncan (Author), Bell, George W. (Contributor), Wiellette, Elizabeth L (Author), Herbolsheimer, Elizabeth (Author), Smith, James C (Author), Yuan, Bingbing B. (Contributor), Sive, Hazel L. (Contributor), Danford, Timothy William (Contributor), Young, Richard A. (Contributor)
• Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
• Format: Article
• Language: English
• Published: BioMed Central Ltd, 2010-10-05T20:08:23Z.
• Subjects: Article
• Online Access: Get fulltext

We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
Wellcome Trust
European Molecular Biology Organization
National Institutes of Health (Grant HG002668) (Grant DK070813) (Grant 2RO1 HD33472) (Grant 5RO1 MH59942)