Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

• Main Authors: Park, YongKeun (Contributor), Yamauchi, Toyohiko (Contributor), Choi, Wonshik (Contributor), Dasari, Ramachandra Rao (Contributor), Feld, Michael S. (Contributor)
• Other Authors: Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Physics (Contributor), Massachusetts Institute of Technology. Spectroscopy Laboratory (Contributor), Feld , Michael S. (Contributor)
• Format: Article
• Language: English
• Published: Optical Society of America, 2010-03-10T14:38:23Z.
• Subjects: Article
• Online Access: Get fulltext

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
Hamamatsu Corporation
National Science Foundation (DBI-0754339)
National Center for Research Resources of the National Institutes of Health (P41-RR02594-18)