Wide-field extended-resolution fluorescence microscopy with standing surface plasmon resonance waves

Wide-field extended-resolution fluorescence microscopy with standing surface plasmon resonance waves

The resolution of conventional SPR imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave surface plasmon resonance fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF p...

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Bibliographic Details
Main Authors: Kim, Yang-Hyo (Contributor), Tang, Wai Teng (Author), So, Peter T. C. (Contributor), Chung, Euiheon (Contributor), Sheppard, Colin J. R. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Mechanical Engineering (Contributor), So, Peter T.C (Contributor)
Format: Article
Language:English
Published: Optical Society of America, 2009-10-19T13:01:02Z.
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Summary:The resolution of conventional SPR imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave surface plasmon resonance fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF provides lateral resolution approaching 100 nm and offers the advantages of significant signal enhancement and background noise reduction. SW-SPRF has the potential for sensitive biomolecular detection, nanoscale imaging, and lithographic applications.

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