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|a Qian, Yong
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|a Piatkevich, Kiryl D
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|a Mc Larney, Benedict
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|a Abdelfattah, Ahmed S
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|a Mehta, Sohum
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|a Murdock, Mitchell H
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|a Gottschalk, Sven
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|a Molina, Rosana S
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|a Zhang, Wei
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|a Chen, Yingche
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|a Wu, Jiahui
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|a Drobizhev, Mikhail
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|a Hughes, Thomas E
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|a Zhang, Jin
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|a Schreiter, Eric R
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|a Shoham, Shy
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|a Razansky, Daniel
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|a Boyden, Edward S
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|a Campbell, Robert E
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|a A genetically encoded near-infrared fluorescent calcium ion indicator
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|b Springer Nature,
|c 2021-11-19T16:19:10Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/138166
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|a © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.
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|a en
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|a Article
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|t 10.1038/S41592-018-0294-6
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|t Nature Methods
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