A genetically encoded near-infrared fluorescent calcium ion indicator

• Main Authors: Qian, Yong (Author), Piatkevich, Kiryl D (Author), Mc Larney, Benedict (Author), Abdelfattah, Ahmed S (Author), Mehta, Sohum (Author), Murdock, Mitchell H (Author), Gottschalk, Sven (Author), Molina, Rosana S (Author), Zhang, Wei (Author), Chen, Yingche (Author), Wu, Jiahui (Author), Drobizhev, Mikhail (Author), Hughes, Thomas E (Author), Zhang, Jin (Author), Schreiter, Eric R (Author), Shoham, Shy (Author), Razansky, Daniel (Author), Boyden, Edward S (Author), Campbell, Robert E (Author)
• Format: Article
• Language: English
• Published: Springer Nature, 2021-11-19T16:19:10Z.
• Subjects: Article
• Online Access: Get fulltext

© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.