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01164 am a22001813u 4500 |
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132925 |
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|a dc
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|a Lavin, Thomas K.
|e author
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|a McGovern Institute for Brain Research at MIT
|e contributor
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|a Jin, Lei
|e author
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|a Wickersham, Ian R.
|e author
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| 245 |
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|a Monosynaptic tracing: a step-by-step protocol
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| 260 |
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|b Elsevier BV,
|c 2021-10-12T16:01:51Z.
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| 856 |
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|z Get fulltext
|u https://hdl.handle.net/1721.1/132925
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|a Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.
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| 520 |
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|a National Institute of Mental Health (Awards U01MH106018, U01MH114829, and U19MH114830)
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|a Article
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|t Journal of Chemical Neuroanatomy
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