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119469 |
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|a Chavez, Alejandro
|e author
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|a Massachusetts Institute of Technology. Institute for Medical Engineering & Science
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Synthetic Biology Center
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|a Collins, James J
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|a Shapiro, Rebecca Sara
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|a Collins, James J.
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|a Collins, James J.
|e author
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|a Shapiro, Rebecca
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|a CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
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|a CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
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|b Nature Publishing Group,
|c 2018-12-07T18:05:24Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/119469
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|a Genetic manipulation of microorganisms has been crucial in understanding their biology, yet for many microbial species, robust tools for comprehensive genetic analysis were lacking until the advent of CRISPR-Cas-based gene editing techniques. In this Progress article, we discuss advances in CRISPR-based techniques for the genetic analysis of genetically intractable microorganisms, with an emphasis on mycobacteria, fungi and parasites. We discuss how CRISPR-based analyses in these organisms have enabled the discovery of novel gene functions, the investigation of genetic interaction networks and the identification of virulence factors.
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|a en_US
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|a Article
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|t Nature Reviews Microbiology
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