Open-source, community-driven microfluidics with Metafluidics

Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture,...

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Bibliographic Details
Main Authors: Kong, David S (Contributor), Thorsen, Todd A. (Contributor), Babb, Jonathan (Contributor), Wick, Scott T. (Contributor), Gam, Jeremy Jonathan (Contributor), Weiss, Ron (Contributor), Carr Sr, Peter A. (Contributor)
Other Authors: Lincoln Laboratory (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2018-09-12T19:28:15Z.
Subjects:
Online Access:Get fulltext
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100 1 0 |a Kong, David S  |e author 
100 1 0 |a Lincoln Laboratory  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Department of Biological Engineering  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science  |e contributor 
100 1 0 |a Kong, David S  |e contributor 
100 1 0 |a Thorsen, Todd A.  |e contributor 
100 1 0 |a Babb, Jonathan  |e contributor 
100 1 0 |a Wick, Scott T.  |e contributor 
100 1 0 |a Gam, Jeremy Jonathan  |e contributor 
100 1 0 |a Weiss, Ron  |e contributor 
100 1 0 |a Carr Sr, Peter A.  |e contributor 
700 1 0 |a Thorsen, Todd A.  |e author 
700 1 0 |a Babb, Jonathan  |e author 
700 1 0 |a Wick, Scott T.  |e author 
700 1 0 |a Gam, Jeremy Jonathan  |e author 
700 1 0 |a Weiss, Ron  |e author 
700 1 0 |a Carr Sr, Peter A.  |e author 
245 0 0 |a Open-source, community-driven microfluidics with Metafluidics 
260 |b Nature Publishing Group,   |c 2018-09-12T19:28:15Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/117729 
520 |a Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. We present Metafluidics, an open-source, community-driven repository that hosts digital design files, assembly specifications, and open-source software to enable users to build, configure, and operate a microfluidic device. We use Metafluidics to share designs and fabrication instructions for both a microfluidic ring-mixer device and a 32-channel tabletop microfluidic controller. This device and controller are applied to build genetic circuits using standard DNA assembly methods including ligation, Gateway, Gibson, and Golden Gate. Metafluidics is intended to enable a broad community of engineers, DIY enthusiasts, and other nontraditional participants with limited fabrication skills to contribute to microfluidic research. 
520 |a National Cancer Institute (U.S.) (Award R01CA173712) 
520 |a National Institute of General Medical Sciences (U.S.) (Award P50GM098792) 
520 |a National Science Foundation (U.S.) (Grant 1124247) 
520 |a National Science Foundation (U.S.) (Grant 1522074) 
520 |a National Science Foundation (U.S.) (Grant 1521925) 
520 |a National Science Foundation (U.S.) (Grant 1521759) 
655 7 |a Article 
773 |t Nature Biotechnology