Bacillus Subtilis SMC Complexes Juxtapose Chromosome Arms as They Travel from Origin to Terminus

• Main Authors: Wang, Xindan (Author), Brandão, Hugo B. (Author), Rudner, David Z. (Author), Le, Tung (Contributor), Laub, Michael T (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor)
• Format: Article
• Language: English
• Published: American Association for the Advancement of Science (AAAS), 2018-07-25T17:19:09Z.
• Subjects: Article
• Online Access: Get fulltext

Structural maintenance of chromosomes (SMC) complexes play critical roles in chromosome dynamics in virtually all organisms, but how they function remains poorly understood. In the bacterium Bacillus subtilis, SMC-condensin complexes are topologically loaded at centromeric sites adjacent to the replication origin. Here we provide evidence that these ring-shaped assemblies tether the left and right chromosome arms together while traveling from the origin to the terminus (>2 megabases) at rates >50 kilobases per minute. Condensin movement scales linearly with time, providing evidence for an active transport mechanism. These data support a model in which SMC complexes function by processively enlarging DNA loops. Loop formation followed by processive enlargement provides a mechanism by which condensin complexes compact and resolve sister chromatids in mitosis and by which cohesin generates topologically associating domains during interphase. Keywords: SMC; ParB; condensin; cohesion; loop extrusion; TAD
National Institutes of Health (U.S.) (Grant GM082899)