Plasma-Treated Microplates with Enhanced Protein Recoveries and Minimized Extractables

• Main Authors: Weikart, Christopher M. (Author), Breeland, Adam P. (Author), Taha, Ahmad H. (Author), Maurer, Brian R. (Author), Martin, Steven P. (Author), Klibanov, Alexander M (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
• Format: Article
• Language: English
• Published: SAGE Publications, 2018-02-15T20:31:38Z.
• Subjects: Article
• Online Access: Get fulltext

SiO2 Medical Products, Inc. (SiO) has developed a proprietary technology that greatly enhances protein recoveries and reduces extractables from commercial microplates used for bioanalytical assays and storage of biologics. SiO technology is based on plasma treatment that chemically modifies the surface of polypropylene with predominantly hydrogen-bond-acceptor uncharged polar groups. The resultant surface resists nonspecific protein adsorption over a wide range of protein concentrations, thereby eliminating the need to passivate (and hence potentially contaminate) the microplates with blocking proteins. High shelf-life stability and cleanliness of the plasma-treated microplates have been demonstrated using five different proteins for two common microplate formats. The protein recovery performance of plasma-treated microplates is found to be higher compared with commercial low-protein-binding microplates. Keywords: microplates; protein adsorption; nonspecific; extractables and leachables; protein recovery; polypropylene plasma treatment