High-resolution interrogation of functional elements in the noncoding genome

High-resolution interrogation of functional elements in the noncoding genome

The noncoding genome affects gene regulation and disease, yet we lack tools for rapid identification and manipulation of noncoding elements. We developed a CRISPR screen using ∼18,000 single guide RNAs targeting > 700 kilobases surrounding the genes NF1, NF2, and CUL3, which are involved in BRAF...

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Bibliographic Details
Main Authors: Fontanillas, P. (Author), Sanjana, Neville E (Contributor), Wright, Jason (Contributor), Zheng, Kaijie (Contributor), Shalem, Ophir (Contributor), Joung, Julia (Contributor), Cheng, Chia-Wei (Contributor), Regev, Aviv (Contributor), Zhang, Feng (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), McGovern Institute for Brain Research at MIT (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor)
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS), 2017-12-13T15:56:11Z.
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