Portable, On-Demand Biomolecular Manufacturing

Synthetic biology uses living cells as molecular foundries for the biosynthesis of drugs, therapeutic proteins, and other commodities. However, the need for specialized equipment and refrigeration for production and distribution poses a challenge for the delivery of these technologies to the field a...

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Bibliographic Details
Main Authors: Pardee, Keith (Author), Nguyen, Peter Q. (Author), Donghia, Nina (Author), Burrill, Devin (Author), Ferrante, Tom (Author), McSorley, Fern R. (Author), Furuta, Yoshikazu (Author), Vernet, Andyna (Author), Lewandowski, Michael (Author), Boddy, Christopher N. (Author), Joshi, Neel S. (Author), Slomovic, Shimyn (Contributor), Lee, Jeongwook (Contributor), Collins, James J. (Contributor)
Other Authors: Institute for Medical Engineering and Science (Contributor), Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Collins, James (Contributor)
Format: Article
Language:English
Published: Elsevier, 2017-09-15T19:56:27Z.
Subjects:
Online Access:Get fulltext
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042 |a dc 
100 1 0 |a Pardee, Keith  |e author 
100 1 0 |a Institute for Medical Engineering and Science  |e contributor 
100 1 0 |a Harvard University-  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Department of Biological Engineering  |e contributor 
100 1 0 |a Collins, James  |e contributor 
100 1 0 |a Slomovic, Shimyn  |e contributor 
100 1 0 |a Lee, Jeongwook  |e contributor 
100 1 0 |a Collins, James J.  |e contributor 
700 1 0 |a Nguyen, Peter Q.  |e author 
700 1 0 |a Donghia, Nina  |e author 
700 1 0 |a Burrill, Devin  |e author 
700 1 0 |a Ferrante, Tom  |e author 
700 1 0 |a McSorley, Fern R.  |e author 
700 1 0 |a Furuta, Yoshikazu  |e author 
700 1 0 |a Vernet, Andyna  |e author 
700 1 0 |a Lewandowski, Michael  |e author 
700 1 0 |a Boddy, Christopher N.  |e author 
700 1 0 |a Joshi, Neel S.  |e author 
700 1 0 |a Slomovic, Shimyn  |e author 
700 1 0 |a Lee, Jeongwook  |e author 
700 1 0 |a Collins, James J.  |e author 
245 0 0 |a Portable, On-Demand Biomolecular Manufacturing 
260 |b Elsevier,   |c 2017-09-15T19:56:27Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/111574 
520 |a Synthetic biology uses living cells as molecular foundries for the biosynthesis of drugs, therapeutic proteins, and other commodities. However, the need for specialized equipment and refrigeration for production and distribution poses a challenge for the delivery of these technologies to the field and to low-resource areas. Here, we present a portable platform that provides the means for on-site, on-demand manufacturing of therapeutics and biomolecules. This flexible system is based on reaction pellets composed of freeze-dried, cell-free transcription and translation machinery, which can be easily hydrated and utilized for biosynthesis through the addition of DNA encoding the desired output. We demonstrate this approach with the manufacture and functional validation of antimicrobial peptides and vaccines and present combinatorial methods for the production of antibody conjugates and small molecules. This synthetic biology platform resolves important practical limitations in the production and distribution of therapeutics and molecular tools, both to the developed and developing world. 
520 |a Defense Threat Reduction Agency (DTRA) (Grant HDTRA1-14-1-0006) 
546 |a en_US 
655 7 |a Article 
773 |t Cell