Creating sub-50 nm nanofluidic junctions in a PDMS microchip via self-assembly process of colloidal silica beads for electrokinetic concentration of biomolecules

• Main Authors: Syed, A. (Author), Song, Y.-A (Author), Mangano, Lauren M. (Contributor), Mao, Pan (Contributor), Han, Joanna M. (Contributor)
• Other Authors: Massachusetts Institute of Technology. Materials Processing Center (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Massachusetts Institute of Technology. Research Laboratory of Electronics (Contributor)
• Format: Article
• Language: English
• Published: Royal Society of Chemistry, The, 2017-08-17T15:06:32Z.
• Subjects: Article
• Online Access: Get fulltext

In this work we describe a novel and simple self-assembly process of colloidal silica beads to create a nanofluidic junction between two microchannels. The nanoporous membrane was used to induce ion concentration polarization inside the microchannel and this electrokinetic preconcentration system allowed rapid concentration of DNA samples by ~1700 times and of protein samples by ~100 times within 5 minutes.
National Institutes of Health (U.S.) (R21 EB008177-01A2)