In Vivo Delivery of Nitric Oxide-Sensing, Single-Walled Carbon Nanotubes

• Main Authors: Iverson, Nicole M. (Contributor), Strano, Michael S. (Contributor), Wogan, Gerald N (Contributor)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor)
• Format: Article
• Language: English
• Published: Wiley Blackwell, 2017-06-09T15:00:16Z.
• Subjects: Article
• Online Access: Get fulltext

Detection of nitric oxide (NO) in vivo by single-walled carbon nanotubes (SWNT) is based on the fluorescent properties of SWNT and the ability of NO to quench the fluorescence signal. Alterations of the signal can be utilized to detect a small molecule in vivo that has not previously been possible by other assay techniques. The protocols described here explain the techniques used to prepare NO-detecting SWNTs and to administer them to mice by both intravenous and subcutaneous routes. These techniques can also be utilized with other SWNT sensors as well as non-SWNT sensor
National Institutes of Health (T32 Training Grant in Environmental Toxicology ES007020)