Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in...

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Main Authors: English, Brian P (Author), Gao, Linyi (Contributor), Suk, Ho-Jun (Contributor), Yoshida, Fumiaki (Author), DeGennaro, Ellen M (Author), Roossien, Douglas H (Author), Cai, Dawen (Author), Tillberg, Paul W. (Contributor), Chen, Fei (Contributor), Piatkevich, Kiryl (Contributor), Zhao, Yongxin (Contributor), Yu, Chih-Chieh (Contributor), Martorell, Anthony (Contributor), Gong, Guanyu (Contributor), Seneviratne, Uthpala Indrajith (Contributor), Tannenbaum, Steven R (Contributor), Desimone, Robert (Contributor), Boyden, Edward (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Massachusetts Institute of Technology. Media Laboratory (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2017-04-28T20:30:04Z.
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