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107914 |
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|a dc
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|a Chavez, Alejandro
|e author
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|a MIT Synthetic Biology Center
|e contributor
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|a Massachusetts Institute of Technology. Department of Biological Engineering
|e contributor
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|a Kiani, Samira
|e contributor
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|a Hall, Richard N.
|e contributor
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|a Beal, Jacob S
|e contributor
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|a Vora, Suhani Deepak
|e contributor
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|a Kowal, Emma J.
|e contributor
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|a Ebrahimkhani, Mohammad Reza
|e contributor
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|a Collins, James J.
|e contributor
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|a Weiss, Ron
|e contributor
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|a Tuttle, Marcelle
|e author
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|a Chari, Raj
|e author
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|a Ter-Ovanesyan, Dmitry
|e author
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|a Qian, Jason
|e author
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|a Pruitt, Benjamin W
|e author
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|a Buchthal, Joanna
|e author
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|a Church, George
|e author
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|a Kiani, Samira
|e author
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|a Hall, Richard N.
|e author
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|a Beal, Jacob S
|e author
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| 700 |
1 |
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|a Vora, Suhani Deepak
|e author
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| 700 |
1 |
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|a Kowal, Emma J.
|e author
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| 700 |
1 |
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|a Ebrahimkhani, Mohammad Reza
|e author
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| 700 |
1 |
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|a Collins, James J.
|e author
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| 700 |
1 |
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|a Weiss, Ron
|e author
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| 245 |
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|a Cas9 gRNA engineering for genome editing, activation and repression
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| 260 |
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|b Nature Publishing Group,
|c 2017-04-06T19:57:41Z.
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| 856 |
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|z Get fulltext
|u http://hdl.handle.net/1721.1/107914
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|a We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
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|a National Human Genome Research Institute (U.S.) (P50 HG005550)
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| 520 |
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|a United States. Department of Energy (DE-FG02-02ER63445)
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| 520 |
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|a Wyss Institute for Biologically Inspired Engineering
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| 520 |
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|a United States. Army Research Office (DARPA W911NF-11-2-0054)
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| 520 |
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|a National Science Foundation (U.S.)
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| 520 |
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|a United States. National Institutes of Health (5R01CA155320-04)
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| 520 |
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|a United States. National Institutes of Health (P50 GM098792)
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| 520 |
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|a National Cancer Institute (U.S.) (5T32CA009216-34)
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| 520 |
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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| 520 |
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|a Harvard Medical School. Department of Genetics
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| 520 |
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|a Defense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006)
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|a en_US
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7 |
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|a Article
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| 773 |
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|t Nature Methods
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