Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

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Transpeptidation catalyzed by ​sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describe...

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Bibliographic Details
Main Authors: Witte, Martin D (Author), Wu, Tongfei (Author), Guimaraes, Carla P (Author), Theile, Christopher S (Author), Blom, Annet E M (Author), Ingram, Jessica R (Author), Kundrat, Lenka (Author), Goldberg, Shalom D (Author), Ploegh, Hidde (Contributor), Li, Zeyang,S.M.Massachusetts Institute of Technology (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor)
Format: Article
Language:English
Published: Springer Nature, 2017-03-07T19:21:07Z.
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