Artificially Engineered Protein Hydrogels Adapted from the Nucleoporin Nsp1 for Selective Biomolecular Transport

• Main Authors: Kim, Minkyu (Contributor), Chen, Wesley George (Contributor), Ribbeck, Katharina (Contributor), Olsen, Bradley D (Contributor), Kang, Jeon Woong (Contributor), Glassman, Matthew J. (Author)
• Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor), Massachusetts Institute of Technology. Laser Biomedical Research Center (Contributor), Glassman, Matthew James (Contributor)
• Format: Article
• Language: English
• Published: Wiley Blackwell, 2017-02-02T20:31:19Z.
• Subjects: Article
• Online Access: Get fulltext

Nucleoporin-like polypeptide (NLP) hydrogels are developed by mimicking nucleoporins, proteins that form gel filters regulating transport into the nucleus. Using protein polymers of a minimal consensus repeat, the NLPs selectively enhance transport of cargo-carrier complexes similar to the natural nuclear pore system. The engineered protein gels additionally have tunable mechanical and transport properties and can be biosynthesized at high yield, making them promising materials for advanced separation technologies.
United States. Defense Threat Reduction Agency (Grant HDTRA1-13-1-0038)
National Institutes of Health (U.S.) (Grant 5-T32-GM008834)
National Institute for Biomedical Imaging and Bioengineering (U.S.) (Grant P41EB015871-28)
MIT Skoltech Initiative