A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen
A highly diverse DNA library coding for ankyrin seven-repeat proteins (ANK-N5C) was designed and constructed by a PCR-based combinatorial assembly strategy. A bacterial melibiose fermentation assay was adapted for in vivo functional screen. We isolated a transcription blocker that completely inhibit...
Main Authors: | Tikhonova, Elena B. (Author), Ethayathulla, Abdul S. (Author), Su, Yue (Author), Hariharan, Parameswaran (Author), Xie, Shicong (Contributor), Guan, Lan (Author) |
---|---|
Other Authors: | Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor) |
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group,
2016-03-28T18:18:29Z.
|
Subjects: | |
Online Access: | Get fulltext |
Similar Items
-
Stucture and accessibility based screening of virtual combinatorial libraries
by: Weaver, Shane George Thomas
Published: (2005) -
Synthesis and Screening of a Combinatorial Peptide Library for Ligands to Target Transferrin: Miniaturizing the Library
by: Brown, Jennifer Marie
Published: (2010) -
Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries
by: Antti Kulmala, et al.
Published: (2019-09-01) -
Combinatorial problems arising from pooling designs for dna library screening
by: Masakazu Jimbo, et al.
Published: (2004-11-01) -
PROFILING THE SUBSTRATE SPECIFICITY OF PROTEIN TYROSINE PHOSPHATASES BY COMBINATORIAL LIBRARY SCREENING
by: Chen, Xianwen
Published: (2011)