A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen

A highly diverse DNA library coding for ankyrin seven-repeat proteins (ANK-N5C) was designed and constructed by a PCR-based combinatorial assembly strategy. A bacterial melibiose fermentation assay was adapted for in vivo functional screen. We isolated a transcription blocker that completely inhibit...

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Bibliographic Details
Main Authors: Tikhonova, Elena B. (Author), Ethayathulla, Abdul S. (Author), Su, Yue (Author), Hariharan, Parameswaran (Author), Xie, Shicong (Contributor), Guan, Lan (Author)
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2016-03-28T18:18:29Z.
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