|
|
|
|
| LEADER |
01912 am a22001933u 4500 |
| 001 |
88611 |
| 042 |
|
|
|a dc
|
| 100 |
1 |
0 |
|a Suhaimi, Siti Hasyimah
|e author
|
| 700 |
1 |
0 |
|a Hasham, Rosnani
|e author
|
| 700 |
1 |
0 |
|a Idris, Mohamad Khairul Hafiz
|e author
|
| 700 |
1 |
0 |
|a Ismail, Hassan Fahmi
|e author
|
| 700 |
1 |
0 |
|a Mohd. Ariffin, Nor Hazwani
|e author
|
| 700 |
1 |
0 |
|a Abdul Majid, Fadzilah Adibah
|e author
|
| 245 |
0 |
0 |
|a Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
|
| 260 |
|
|
|b MDPI AG,
|c 2019-11.
|
| 856 |
|
|
|z Get fulltext
|u http://eprints.utm.my/id/eprint/88611/1/RosnaniHasham2019_OptimizationofUltrasound-AssistedExtractionConditions.pdf
|
| 520 |
|
|
|a Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surfacemethodology (RSM)was performed to develop an extractionmethodwithmaximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21min, ultrasound amplitudes 62A, and solvent composition 70%ethanol inwater. The crude extractwas further fractionated using solid-phase extraction (SPE),where six sequential fractions that varied in polarity (0-100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells.
|
| 546 |
|
|
|a en
|
| 650 |
0 |
4 |
|a TP Chemical technology
|
