Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers

Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia using ISSR and RAPD markers. A total of 50 acce...

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Bibliographic Details
Main Authors: Arif, Muhammad Fauzi (Author), Subositi, Dyah (Author), Sari, Aniska Novita (Author), Aristya, Ganies Riza (Author), Lesmana, Indra (Author), Kasiamdari, Rina Sri (Author)
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia, 2020.
Online Access:Get fulltext
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100 1 0 |a Arif, Muhammad Fauzi  |e author 
700 1 0 |a Subositi, Dyah  |e author 
700 1 0 |a Sari, Aniska Novita  |e author 
700 1 0 |a Aristya, Ganies Riza  |e author 
700 1 0 |a Lesmana, Indra  |e author 
700 1 0 |a Kasiamdari, Rina Sri  |e author 
245 0 0 |a Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers 
260 |b Penerbit Universiti Kebangsaan Malaysia,   |c 2020. 
856 |z Get fulltext  |u http://journalarticle.ukm.my/15700/1/49_01_07.pdf 
520 |a Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia using ISSR and RAPD markers. A total of 50 accessions of A. paniculata were used, DNA was amplified using six ISSR and six RAPD primers, generating 61 loci and 43 loci respectively. Genotype data scoring resulted in the percentage of polymorphism, Polymorphic Information Content, Shannon's Information Index and unbiased expected heterozygosity. The genetic variation between the locations was measured with Principal Coordinate Analysis (PCoA) and pairwise Nei's unbiased genetic distance. The data from this study revealed that the variation and diversity from accessions within the same island were low. The genetic variation was higher between accessions from different islands. Analysis of molecular variance (AMOVA) was used to calculate the distribution of the variation between and within the A. paniculata population. The results showed the variation derived from the same location was 34% and from a different location was 66%. Genetic diversity provided information to help the further development and cultivation of A. paniculata in Indonesia. 
546 |a en