Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes

Detection of Leptospira species in environmental samples by amplification of 16S rRNA and rpoβ genes

This study attempted to identify and determine distribution of Leptospira spp. in environmental samples using 16S rRNA and rpoβ genes amplification. The samples were collected from high risk areas in Selangor, Malaysia. A total of 105 environmental samples consisting of soil and water were subjected...

Full description

Bibliographic Details
Main Authors:	Nurul Farhana Zulkifli (Author), Steffi Julan Wan (Author), Vasantha Kumari Neela (Author), Chee, Hui Yee (Author), Siti Norbaya Masri (Author), Mazen M. Jamil Al-Obaidi (Author), Mohd Nasir Mohd Desa (Author)
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia, 2018-08.
Online Access:	Get fulltext

Similar Items

Leptospira interrogans and Leptospira kirschneri are the dominant Leptospira species causing human leptospirosis in Central Malaysia.
by: Noraini Philip, et al.
Published: (2020-03-01)

Comparative analysis of current diagnostic PCR assays in detecting pathogenic Leptospira isolates from environmental samples
by: May-Ling Yap, et al.
Published: (2019-01-01)

Isolation and molecular characterization of Leptospira interrogans and Leptospira borgpetersenii from small mammals in Selangor wet markets
by: N. Bahtiar Affendy, et al.
Published: (2020-12-01)

Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
by: Lenice Roteia Cardoso Jung, et al.
Published: (2015-06-01)

TYPING OF LEPTOSPIRA SPP. STRAINS BASED ON 16S rRNA
by: Yu. V. Ostankova, et al.
Published: (2016-02-01)

Comparative evaluation of PCR amplification of RLEP, 16S rRNA, rpoT and Sod A gene targets for detection of M. leprae DNA from clinical and environmental samples
by: Ravindra P Turankar, et al.
Published: (2015-01-01)

A comparison of rpoB and 16S rRNA as markers in pyrosequencing studies of bacterial diversity.
by: Michiel Vos, et al.
Published: (2012-01-01)

Molecular Methods for Identification of Acinetobacter Species by Partial Sequencing of the rpoB and 16S rRNA Genes
by: Azar Dokht Khosravi, et al.
Published: (2015-07-01)

The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB
by: Yuko Kazumi, et al.
Published: (2012-01-01)

Leptospiral Culture without 5’-Fluorouracil Revealed Improved <i>Leptospira</i> Isolation from Febrile Patients in North-Eastern Malaysia
by: Amira Wahida Mohamad Safiee, et al.
Published: (2020-02-01)

Genomic data of Leptospira interrogans HP358 isolated from rodent captured from the human leptospirosis suspected areas of Selangor state, Malaysia
by: Noraini Philip, et al.
Published: (2021-08-01)

Isolation and identification of Leptospira species from bovines by rpoB and LipL41 genes based phylogenetic analysis
by: Shivaraj Murag, et al.
Published: (2021-01-01)

Identification of Pathogenic Leptospira in Rat and Shrew Populations Using rpoB Gene and Its Spatial Distribution in Boyolali District
by: Dyah Widiastuti, et al.
Published: (2016-08-01)

Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics.
by: Antony T Vincent, et al.
Published: (2019-05-01)

Leptospira interrogans and leptospira kirschneri are the dominant leptospira species causing human leptospirosis in central Malaysia
by: Affendy, N.B, et al.
Published: (2020)

Seroprevalence of Leptospira infection in occupational risk groups in North Khorasan province, Iran
by: Seyed-Ahmad Hashemi, et al.
Published: (2021-01-01)

Contribution of RpoS and RpoE to bile adaptation in Vibrio parahaemolyticus
by: Chi-Ying Wang, et al.
Published: (2014)

Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic Leptospira
by: Ahmed Ahmed, et al.
Published: (2014-05-01)

Chitobiose utilization in <it>Borrelia burgdorferi </it>is dually regulated by RpoD and RpoS
by: Rhodes Ryan G, et al.
Published: (2009-05-01)

Application of a Loop-Mediated Isothermal Amplification Method for the Detection of Pathogenic Leptospira
by: Yun-Hsiu Hsu, et al.

Phylogenetic relationship of Ornithobacterium rhinotracheale isolated from poultry and diverse avian hosts based on 16S rRNA and rpoB gene analyses
by: Inês M. B. Veiga, et al.
Published: (2019-02-01)

rpoB Targeted Loop-Mediated Isothermal Amplification (LAMP) Assay for Consensus Detection of Mycobacteria Associated With Pulmonary Infections
by: Simon Grandjean Lapierre, et al.
Published: (2018-11-01)

Correction: Role of Acetyl-Phosphate in Activation of the Rrp2-RpoN-RpoS Pathway in.
by: Haijun Xu, et al.
Published: (2010-12-01)

16S ribosomal DNA amplification for phylogenetic study and specific detection and differentiation of leptospira species
by: Lu,Meng-Shian, et al.
Published: (1996)

Combined PCR and MAT improves the early diagnosis of the biphasic illness leptospirosis.
by: Noraini Philip, et al.
Published: (2020-01-01)

Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
by: V. Kalidasan, et al.
Published: (2020-01-01)

Selective Amplification of SPR Biosensor Signal for Recognition of rpoB Gene Fragments by Use of Gold Nanoparticles Modified by Thiolated DNA
by: M. Matsishin, et al.
Published: (2017-04-01)

Cloning, Identification, and Characterization of the rpoS-Like Sigma Factor rpoX from Vibrio alginolyticus
by: Jing-jing Zhao, et al.
Published: (2009-01-01)

Role of acetyl-phosphate in activation of the Rrp2-RpoN-RpoS pathway in Borrelia burgdorferi.
by: Haijun Xu, et al.
Published: (2010-09-01)

Activation of the RpoN-RpoS regulatory pathway during the enzootic life cycle of <it>Borrelia burgdorferi</it>
by: Ouyang Zhiming, et al.
Published: (2012-03-01)

Regulatory Roles of the Sigma Factors RpoS and RpoN in the Pathogenesisof Vibrio vulnificus YJ016
by: Chun-Liang, et al.
Published: (2005)

Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies.
by: Patrick D Schloss, et al.
Published: (2011-01-01)

EFFICIENCY OF REAL-TIME PCR FOR 18S rRNA AMPLIFICATION OF SORBUS DOMESTICA, L.
by: Petronela Poláčeková, et al.
Published: (2012-10-01)

Reducing the Effects of PCR Amplification and Sequencing Artifacts on 16S rRNA-Based Studies
by: Schloss, Patrick D., et al.
Published: (2012)

Optimization of Annealing Temperature for Amplification of 507 bp fragment of rpoB Gene of Clinical Multidrug-Resistant Mycobacterium tuberculosis Isolate 86
by: E.P. Rusyanthini, et al.
Published: (2015-07-01)

The regulation of rpoBC in Escherichia coli
by: Morgan, Brian Alexander
Published: (1986)

Combined PCR and MAT improves the early diagnosis of the biphasic illness leptospirosis
by: Noraini Philip, et al.
Published: (2020-01-01)

Identification of rpoH gene and promoter analysis of the rpoD gene from Xanthomonas campestris pv. campestris.
by: Huang, Lan-Hui, et al.
Published: (1997)

Characterization of the structure and expression of the Euglena gracilis chloroplast rpoC1 and rpoC2 gene loci.
by: Radebaugh, Catherine Ann, 1956-
Published: (1990)

Characterization of a Group B Streptococcus infection based on the demographics, serotypes, antimicrobial susceptibility and genotypes of selected isolates from sterile and non-sterile isolation sites in three major hospitals in Malaysia
by: Mohd E.S. Suhaimi, et al.
Published: (2017-01-01)

Cannot write session to /tmp/vufind_sessions/sess_tdsliur1ajrp43dlupdknlba9n