Intracerebroventricularly Transplanted Embryonic Neuronal Tissue from Inflammatory-Resistant F344/N Rats Decreases Acoustic Startle Responses in Inflammatory-Susceptible Lew/N Rats

• Main Authors: B. Misiewicz, M. Poltorak, M. Gomez, J.R. Glowa, P.W. Gold, E.M. Sternberg
• Format: Article
• Language: English
• Published: SAGE Publishing 1996-03-01
• Series: Cell Transplantation
• Online Access: https://doi.org/10.1177/096368979600500217

Recently, we have shown that intracerebral transplantation of fetal F344/N hypothalamic tissue into LEW/N rats converts the LEW/N inflammatory-susceptible phenotype into an inflammatory-resistant phenotype in LEW/N hosts. Because LEW/N rats also exhibit relatively high acoustic startle responses (ASRs) compared to F344/N rats, in the present study we examined the effects on ASR of transplantation of F344/N hypothalamic tissue into the third ventricle of LEW/N rats. Dissected neuronal tissue from F344/N rats (Day E15-16) was implanted into the third ventricle of LEW/N rats. After 4 wk of postoperative survival, the animals' responses to acoustic startle stimuli were tested. Compared to naive and sham-operated animals, LEW/N rats transplanted with hypothalamic tissue exhibited significant decreases in ASR amplitudes. A similar decrease in ASR amplitude was observed in the group of LEW/N rats transplanted with embryonic striatal tissue. Our results indicate that the third ventricular neuronal grafts may modulate behavioral responses in the LEW/N rats. Although the mechanism of this effect is unknown, these studies suggest that intracerebral neuronal transplantation is a viable method with which to explore mechanisms of behavioral, neuroendocrine, and inflammatory response associations.