Complete Genome Sequence and Characterization of a Polyethylene Biodegradation Strain, <i>Streptomyces Albogriseolus</i> LBX-2

• Main Authors: Huanhuan Shao, Meiju Chen, Xueting Fei, Ronglin Zhang, Yue Zhong, Weimin Ni, Xiang Tao, XinYi He, Erliang Zhang, Bin Yong, Xuemei Tan
• Format: Article
• Language: English
• Published: MDPI AG 2019-09-01
• Series: Microorganisms
• Subjects: <i>Streptomyces albogriseolus</i> LBX-2; polyethylene biodegradation; genome; synteny comparison
• Online Access: https://www.mdpi.com/2076-2607/7/10/379

A bacterial strain, <i>Streptomyces albogriseolus</i> LBX-2, was isolated from a soil sample in Chengdu, China. <i>S. albogriseolus</i> LBX-2 is an aerobic and Gram-positive microorganism that is capable of using the polyethylene as the sole carbon source. Results of scanning electron microscopy and tensile tests indicated that <i>S. albogriseolus</i> LBX-2 could cause the damages to polyethylene (PE). Suspension culture of LBX-2 resulted in the weight loss in the PE powder over a 15-day period. The bacterial growth curve assay clearly demonstrated the utilization of n-hexadecane and n-octadecane for the strain LBX-2. Phylogenetic analysis showed that it was grouped in the same clade as <i>S. albogriseolus</i> belonging to <i>Streptomyces</i>. The complete genome of strain LBX-2 consists of a chromosome of 7,210,477 bp and a linear plasmid of 336,677 bp. Compared with other strains of <i>Streptomyces</i>, the genome size of <i>S. albogriseolus</i> LBX-2 was smaller than the average but its guanine and cytosine content (72.47%) was higher than the others. The Non-Redundant Protein Database (NR), Kyoto Encyclopedia of Genes and Genomes (KEGG), SwissProt, Gene Ontology (GO) and Clusters of Orthologous Groups (COG) annotations provided information on the specific functions of encoded proteins. A total of 21 monooxygenase and 22 dioxygenase genes were found in its genome. Synteny comparison with the genome of <i>Streptomyces coelicolor</i> A3(2) revealed a low overall genetic diversity between them. This study provides valuable information to reveal the underlying mechanisms on PE degradation by <i>S. albogriseolus</i> LBX-2.