ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data

ReadqPCR and NormqPCR: R packages for the reading, quality checking and normalisation of RT-qPCR quantification cycle (Cq) data

<p>Abstract</p> <p>Background</p> <p>Measuring gene transcription using real-time reverse transcription polymerase chain reaction (RT-qPCR) technology is a mainstay of molecular biology. Technologies now exist to measure the abundance of many transcripts in parallel. Th...

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Bibliographic Details
Main Authors: Perkins James R, Dawes John M, McMahon Steve B, Bennett David LH, Orengo Christine, Kohl Matthias
Format: Article
Language:English
Published: BMC 2012-07-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/13/296

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