AMPLICATION OF 0.7KB FRAGMENT KATG GENE FROM CLINICAL MULTI DRUG RESISTANT TUBERCULOSIS ISOLATE IN BALI

• Main Authors: A. W. Dwiputri, K. Ratnayani, S. C. Yowani
• Format: Article
• Language: English
• Published: Universitas Udayana 2014-06-01
• Series: Indonesia Journal of Biomedical Science
• Subjects: amplification, katg gene, mdr-tb, 0.7 kb
• Online Access: https://ijbs-udayana.org/index.php/ijbs/article/view/118

During last decade has seen a particular increase in the occurrence of drug-resistant of tuberculosis (DR-TB) and multi-DR strains, such as Isoniazid (INH) resistant strains of <em>M. tuberculosis</em>.  INH resistance is more frequently associated with mutations in the <em>katG</em> gene. Detection of <em>katG</em> gene mutations can be performed by PCR technique, followed by sequences. The aim of this study is to amplify <em>kat</em>G gene region (0,7 Kb) from clinical isolate of MDR-TB in Bali. DNA isolation for PCR was done by Boom method and <em>katG </em>gene amplification was performed under the following conditions: predenaturation at 95⁰C for 15 min; fourty cycles of denaturation at 94⁰C for 1 min, annealing at 56⁰C for 1 min, extension at 72⁰C for 2 min; final extension at 72⁰C for 10 min. The amplicons were detected by 1.5% agarose gel electrophoresis and showed a specific band size at 0.7 kb. This suggests that the fragment of <em>katG</em> gene has been successfully amplified in these areas.