A PCR-Based Method for RNA Probes and Applications in Neuroscience

A PCR-Based Method for RNA Probes and Applications in Neuroscience

In situ hybridization (ISH) is a powerful technique that is used to detect the localization of specific nucleic acid sequences for understanding the organization, regulation, and function of genes. However, in most cases, RNA probes are obtained by in vitro transcription from plasmids containing spe...

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Bibliographic Details
Main Authors: Ruifang Hua, Shanshan Yu, Mugen Liu, Haohong Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-05-01
Series:Frontiers in Neuroscience
Subjects:
in situ hybridization
RNA probe
PCR
transgenic mice
immuno-fluorescence
retrograde tracing
Online Access:http://journal.frontiersin.org/article/10.3389/fnins.2018.00266/full

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