Evaluation of a Method for Nitrotyrosine Site Identification and Relative Quantitation Using a Stable Isotope-Labeled Nitrated Spike-In Standard and High Resolution Fourier Transform MS and MS/MS Analysis

Evaluation of a Method for Nitrotyrosine Site Identification and Relative Quantitation Using a Stable Isotope-Labeled Nitrated Spike-In Standard and High Resolution Fourier Transform MS and MS/MS Analysis

The overproduction of reactive oxygen and nitrogen species (ROS and RNS) can have deleterious effects in the cell, including structural and possible activity-altering modifications to proteins. Peroxynitrite is one such RNS that can result in a specific protein modification, nitration of tyrosine r...

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Bibliographic Details
Main Authors: Kent W. Seeley, Alison R. Fertig, Craig P. Dufresne, Joao P. C. Pinho, Stanley M. Stevens Jr.
Format: Article
Language:English
Published: MDPI AG 2014-04-01
Series:International Journal of Molecular Sciences
Subjects:
nitration
oxidative stress
immonium ion
high resolution mass spectrometry
liquid chromatography (LC)-MS/MS
FTMS
Online Access:http://www.mdpi.com/1422-0067/15/4/6265

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